Trx-1 over-expression attenuates oxidative stress injury in MPP+ -induced PC12 cells by regulating NF-κB signaling pathway

AIM:To investigate the inhibitory effect of thioredoxin 1 (Trx-1) over-expression on oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP⁺)-induced rat pheochromocytoma PC12 cells by regulating NF-κB signaling pathway.METHODS:The PC12 cells were damaged by treatment with MPP⁺ at 1, 3 and 5 mmol/L, and the optimal concentration of 3 mmol/L was selected. The cell viability was measured by MTT assay. The oxidative stress indexes lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the cell culture supernatant were detected, and the protein expression of Trx-1 was determined by Western blot. Lentiviral infection with Ad-Trx-1-GFP sequence was used to establish a model of MPP⁺-treated PC12 cells with Trx-1 over-expression. The effects of Trx-1 over-expression on the cell viability, oxidative stress responses and NF-κB signaling pathway were determined by MTT assay, commercial kits and Western blot. The effects of phorbol 12-myristate 13-acetate (PMA), an activator of NF-κB signaling pathway, on the viability and oxidative stress of PC12 cells were observed. The NF-κB signaling pathway inhibitor pyrrolidine dithiocarbamate (PDTC) was used in MPP⁺-treated PC12 cells with Trx-1 over-expression, and the cell viability and oxidative stress responses were measured. RESULTS:The viability of PC12 cells, SOD activity in the supernatant and protein expression of Trx-1 were decreased, while LDH activity and MDA content in the supernatant were increased significantly by treatment with MPP⁺ at 1, 3 and 5 mmol/L. The effect of MPP⁺ at 3 mmol/L and 5 mmol/L was significantly greater than that at 1 mmol/L (P<0.05), and no significant difference between 3 mmol/L and 5 mmol/L was observed (P>0.05). The inhibitory effect of MPP⁺ on the viability of PC12 cells, and the oxidative stress injury and activation of NF-κB signaling pathway induced by MPP⁺ were significantly attenuated by over-expression of Trx-1. The inhibitory effect of MPP⁺ on the viability of PC12 cells and the oxidative stress injury induced by MPP⁺ were promoted by the activation of NF-κB signaling pathway, while the protective effects of Trx-1 over-expression on the MPP⁺-treated PC12 cells were enhanced by the inhibition of NF-κB signaling pathway. CONCLUSION:Over-expression of Trx-1 protects MPP⁺-treated PC12 cells from oxidative stress injury by regulating NF-κB signaling pathway.     

鲁西南地区10个小麦品种丰产性和稳产性分析

为更科学的分析评价鲁西南地区主栽的10个小麦品种丰产性和稳定性,本研究利用联合方差分析、线性回归分析和AMMI模型对 3个试验地点产量数据进行分析。结果表明,菏麦20、济麦22和周麦22丰产性和稳定性表现优异,周麦18、烟农999和山农20属于丰产性较好稳产性一般的品种,良星99和山农28属于稳产性好丰产性一般的品种,青农2号稳产性较好丰产性较差,但矮抗58丰产性和稳产性均较差但在E3具有特定区域适应性。     

怀化地区强降水过程中的GPS可降水量特征分析

利用改进的天顶静力延迟（ZHD）模型和本地化水汽权重平均温度（Ts）模型反演怀化地区GPS可降水量（GPS-PWV），并结合自动气象站逐小时资料分析了2017年怀化地区大气水汽变化及汛期14次暴雨以上降水过程的GPS-PWV演变特征。结果表明：GPS-PWV可较好反映怀化地区大气水汽的变化特征。怀化地区可降水量-气压（PWV-P）分布月变化特征明显，冬季PWV较低且变化范围较小，降水发生时气压较夏季平均高14.75 hPa；春季PWV逐步增大，降水发生时气压较冬季有所降低；夏季PWV为全年最高，降水发生时气压则降至全年最低值；秋季PWV-P数据逐渐分散并向可降水量低值区移动，分布情况逐步趋近冬季。2017年汛期怀化地区14次强降水过程中PWV均高于各月均值，最大小时降水量与最大PWV存在较好对应关系；降水开始前，PWV出现较明显上升，且多伴随气压较明显下降，可为局地强降水短临预警提供较好参考。     

三种水稻栽培模式对主要害虫种群发生动态的影响

轻简化和机械化是现代农业发展的趋势。本文于2 0 1 3 —2 0 1 5 年连续进行了常规人工栽插、机械直播、机械栽插等3种栽培模式对水稻主要害虫种群发生动态的影响研究,旨在为农业轻简、机械化模式的进一步推广提供依据。结果表明,3种栽培模式对稻飞虱、稻纵卷叶螟种群发生的影响差异不明显。比较3 种栽培模式下的水稻螟虫(二化螟为主, 大螟较少) 发生和为害可知,机械直播田的螟虫前期株为害率较高,且后期仍维持较高的为害率,明显高于人工栽插和机械插秧田(p<0.05)。分析原因,很可能是机械直播水稻暴露在大田的时间要稍长于栽插模式(机械插秧和人工栽插),有利于二化螟的提前入田为害。因此在推广机械直播时,应考虑调整播期,尽可能地避开或减少螟虫为害。     

固相萃取离子色谱法快速测定烟草中钾、钠、钙、镁和氨的研究

采用带固相萃取净化功能的样品萃取瓶超声萃取烟草样品,样品萃取液用石墨化炭黑球固相萃取净化。然后以IonPacCS12A阳离子交换色谱柱(3 ′ 150 mm,5 μm)为固定相,30 mM甲基磺酸为流动相,淋洗液流速1.0 mL/min,K+、Na+、Mg2+、Ca2+、和NH4+ 5种阳离子在6.0 min内可达到完全分离。采用自主设计的样品萃取瓶,可不经样品转移就完成样品的萃取、净化和过滤。和常规方法相比,操作流程得到简化,效率大幅度提高,灵敏度和回收率高、精密度好。为基因编辑素材钾、钠、钙、镁和氨的快速测定提供了高通量分析方法。     

甜菜抗褐斑病种质资源的研究现状

甜菜抗褐斑病种质资源是甜菜种质资源中具有褐斑病抗性基因并且能稳定传递给子代的种质资源,是甜菜抗褐斑病育种工作的物质基础,也是农业可持续发展的战略资源。甜菜抗褐斑病种质资源研究的主要内容包括对甜菜品种的收集、鉴定与评价、保存、利用以及抗性遗传基因的克隆及利用。为了使人们更加清晰的了解甜菜抗褐斑病种质资源的研究状况。本文综述了甜菜抗褐斑病种质资源的收集、鉴定与评价、保存、利用及分子方面的研究,并对我国未来种质资源的研究作出展望。以期为以后甜菜抗褐斑病种质资源的研究提供方向。     

利用正交实验设计研究磺胺甲恶唑在渔业水体中的消解动态规律

为了探究磺胺甲恶唑(sulfamethoxazole,SMZ)在渔业水体中的自然降解规律,本试验选取了养殖水环境中常见的因素(温度、pH和敞蔽条件),通过正交实验探究这三个因素对SMZ在自然养殖水体中消解动态的影响。试验初期各处理SMZ浓度保持一致,实验周期内定期取水样检测水体中SMZ的浓度变化,符合一级反应动力学方程,计算各处理中SMZ药物的半衰期和消除率。结果表明,18个处理组SMZ的半衰期及其消除率变化范围为4.88-12.89d,28%-77%。方差分析得出：在实验水平范围内,温度、pH因素对SMZ的消解结果有显著影响,但敞蔽的影响较小,因此提高养殖水体高温度、调节酸碱性pH等条件均有利于促进SMZ的降解。     

14份小黑麦资源在青海民和地区的适应性试验

[目的]为获得适宜在青海民和地区种植的小黑麦品种,[方法]在当地进行国内外小黑麦品种的适应性试验,测定其生育期、株高、出苗率、发芽等指标。[结果]结果表明：国内的小黑麦品种比国外的品种生育期短2-10 d；株高相比较而言“Pingpong”最高,达164.3cm,其次是“中饲1048”(158.07cm)和“中饲3241”(153.50cm)；出苗率“中饲1048”最高,为60.67%；综合发芽指标及千粒重分析表明, “中饲1048”最高,“中饲3241”次之,在该地区表现出良好的适应性。[结论]因此,“Pingpong”、“中饲1048”和“中饲3241”三个品种可以作为该地区种植的推荐品种。     

农业产业化联合体建设及人才培养探讨

农业产业化联合体是一种新型农业生产经营组织联盟,它对推动农业产业化发展、带动农民脱贫致富、促进三产融合发展起到极其重要作用。本文论述我国农业产业化合体建设及其人才培养现状,分析农业产业化合体建设及其人才培养面临的主要困难与问题,提出支持农业产业化合体建设及其人才培养的对策建议。